Keanu Reeves

Thomas "Neo" Anderson (voice)

Carrie-Anne Moss

Trinity (voice)

Clayton Watson

The Kid (voice)

Pamela Adlon

Jue ("Final Flight of the Osiris") / Manabu ("Beyond") (voice)

Melinda Clarke

Alexa ("Matriculated") (voice)

John DiMaggio

Crew Man ("Final Flight of the Osiris") / Kaiser ("Program") (voice)

Kevin Michael Richardson

Thadeus ("Final Flight of Osiris") / Cop ("Kid's Story") / Agent #2 ("World Record")

Tom Kenny

Operator ("Final Flight of Osiris")

Rick Gomez

Pilot ("Final Flight of Osiris")

Tara Strong

Crew Woman ("Final Flight of Osiris") / Nurse ("World Record") / Misha ("Beyond")

Bette Ford

Old Woman ("Final Flight of Osiris")

Julia Fletcher

The Instructor ("The Second Renaissance Part I & II") / Narrator ("World Record")

Dane A. Davis

01 Versatran Spokesman ("The Second Renaissance Part I & II")

Debi Derryberry

Kid ("The Second Renaissance Part I & II")

Jill Talley

Mother ("The Second Renaissance Part I & II")

Dwight Schultz

Additional Voices ("The Second Renaissance Part I & II") / Monaka ("Matriculated")

James Arnold Taylor

Additional Voices ("The Second Renaissance Part I & II") / Ash ("A Detective Story") / Raul ("Matriculated")

John DeMita

Teacher ("Kid's Story")

Hedy Burress

Cis ("Program") / Yoko ("Beyond")

Phil LaMarr

Duo ("Program")

Victor Williams

Dan ("World Record")

John Wesley

Dan's Dad ("World Record")

Alex Fernandez

Tom ("World Record")

Allison Smith

Reporter ("World Record")

Matt McKenzie

Agent #1 ("World Record") / Agent ("Beyond") / Agent ("A Detective Story")

Tress MacNeille

Housewife ("Beyond") / Kenny ("Beyond")

Kath Soucie

Pudgy ("Beyond") / Masa ("Beyond") / Sara ("Beyond")

Terrence 'T.C.' Carson

Clarence ("A Detective Story")

Rodney Saulsberry

Chyron ("Matriculated")

Olivia d'Abo

Rox ("Matriculated")

Jack Fletcher

Sandro ("Matriculated")

Akio Otsuka

Thadeus (voice)